gallic acid mass spectrum

Gallic acid esters, such as tannins, catechin gallates and aliphatic gallates are potent antioxidants in vitro. O–H stretch from 3300-2500 cm-1; C=O stretch from 1760-1690 cm-1; C–O stretch from 1320-1210 cm-1; O–H bend from 1440-1395 and 950-910 cm-1; Figure 11. shows the spectrum of hexanoic acid … Gallic acid was isolated from EtOAc by another method previously reported by Ben Saad and Kim, 2015 . mzCloud ‒ Free Online Mass Spectrometry Database It is commonly used in the pharmaceutical industry. 240 Natural Product Communications Vol. The fragment of the [M-H-CO 2 ] − ion at m/z 125 was shown in the MS–MS experiment. Based on the previous studies on its metabolic fate, gallic acid will be metabolized through decarboxylation, O-methylation, sulfation, and glucuronidation reactions in rats [18, 19]. This enzyme has a molecular mass of approximately 320 kDa and consists of six identical subunits. Mass spectra were recorded in negative and positive ionization modes with the ion source voltage of 4.95 kV, the source current of 2.61 μA, the capillary temperature of 275 °C, the capillary voltage of 50 V, the sheath gas pressure of 137 KPa and auxiliary gas pressure of 13.9 KPa. Gallic acid is a phenolic compound also known as 3,4,5-trihydroxybenzoic acid. GA have been reported to have potent free radical scavenging and anti-oxidative activities [10] , [11] and therefore the study of the mechanism of action of GA has received much … This is consistent with three hydrogens providing hyperfine splitting. The resulting spectrum showed the presence of three initiator species, at m/z ratios of 498, 514, and 530, formed by a reaction between tertiary amine and epoxides precursors, i.e., di-, tri-, and tetra-glycidyl ether of gallic acid, respectively (Fig. Reference data were obtained primarily from the PubChem database.. Three dimensional molecular rendering uses Jmol.. InChI string and atom numbering calculated using ALATIS (Hesam Dashti, William M. Westler, John L. Markley, Hamid R. Eghbalnia, "Unique identifiers for small molecules enable rigorous labeling of … Use this link for bookmarking this species for future reference. After introduction of gallic acid QCG (Fig. 4. Gallic acid (GA), an endogenous plant phenol, is a naturally abundant plant compound in vegetables, tea, grapes, berries, as well as wine –. As the precursor of tannin, gallic acid presented an [M-H] − ion at m/z 169 in the full scan mass spectrum. Gallic acid is found both free and as part of tannins. Gallic acid fails to attenuate isoproterenol-induced myocytolysis[3]. 1B) has a peak of symmetric protons in the 1 H NMR spectrum the formation of a new amide and ester bonds in the IR spectrum is seen in the region of 1550−1700 cm −1. Gallic acid: Description: Gallic acid is an organic acid, also known as 3,4,5-trihydroxybenzoic acid, found in gallnuts, sumac, witch hazel, tea leaves, oak bark, and other plants. Gallic acid decarboxylase … Communication and Mass Media - ... method for analysis of Gallic acid gallic acid Subject Category: ... methanol: formic acid (3: 3: 0.2: 0.8 v/v) system was developed as a mobile phase. Gallic acid, catechin and cinnamic acid and related derivatives originated form rhubarb. 137.0244 was observed by loss of gallic acid (GOH, Δm=170) and water (H2O, Δm=18) molecules.Ion at m/z 137.0244 underwent CO2 loss to generate ion at m/z 93.0346. The MALDI-TOF spectrum showed a peak series exhibiting a mass increment of 288 Da, reflecting the variation in the number of catechin/epicatechin units. CAS=121-79-9, Molecular Formula=C 10 H 12 O 5, Molecular Weight (g/mol)=212.2, MDL Number=MFCD00002196, InChI Key=ZTHYODDOHIVTJV-UHFFFAOYSA-N, Synonym=propyl gallate,n-propyl gallate,progallin p,tenox pg,nipagallin p,gallic acid, propyl ester,gallic acid propyl ester,nipa 49,benzoic acid, 3,4,5-trihydroxy-, … Spectrum analysis showed the same Rf values and spectrum pattern of standard and sample. Gallic acid chloride was synthesized by the dropwise addition, over a period of 0.25hr, of 3.16ml (44.3mmol) of thionyl chloride to a solu-tion of 5.03g (29.6mmol) of gallic acid and three drops of dimethylformamide in 20ml of anhydrous tetrahydrofuran (THF) maintained near … After 18 h treatment with Gallic acid, the number of viable neutrophils is dramatically decreased from 40.3% to 27.7%, highly comparable with 26.4% for untreated neutrophils. Gallic acid glycoside was enzymatically synthesized by using dextransucrase and sucrose from gallic acid. In addition to quercetin, the presence of gallic acid, The data also suggest CAS … Our results suggest CAS contains polyphenols with strong α-amylase inhibitory activity. Last modification occurred on 10/17/2016 1:12:45 PM. The results indicate that ellagic acid, gallic acid and punicalagin may be the compounds responsible for the anti-inflammatory potential of P. granatum. In the pH range between 7-10, the spectrum of the gallate free radical is a doublet of triplets (a H = 1.00 G, a H = 0.23 G, a H = 0.28 G). Structure, properties, spectra, suppliers and links for: Gallic acid, 149-91-7. After purification by butanol partitioning and preparative HPLC, gallic acid glucoside was detected at m/z 355 (C13, H16, O10, Na)+ by matrix-assisted laser desorption ionization time-of-flight mass spectrometry. LC-MS Waters was used for mass detection. Go To: Top, Mass spectrum (electron ionization), Gas Chromatography, References Data from NIST Standard Reference Database 69: NIST Chemistry WebBook The National Institute of Standards and Technology (NIST) uses its best efforts to deliver a high quality copy of the Database and to verify that the data contained therein … 5995-86-8. 2.4.1. As an ACS grade quality reagent, its chemical specifications are the de facto standards for chemicals used in many high-purity … In Vivo 3,4,5-Trihydroxybenzoic acid monohydrate. The fatty acid composition was identified by a mass spectrum database (NIST Library, mass spectral search program, version … Gallic acid is an antioxidant which can inhibit both COX-2[1]. The yield of gallic acid … In the present work, gallic acid as a trapping agent of blood formaldehyde was investigated by liquid chromatography/tandem mass spectrometry (LC/MS/MS) and neutral loss scan. The molecular structure of gallic acid and catechin were predicted in Fig. Figure 5: Mass spectra of (A) Catechin (Cf 1) and (B) Gallic acid (Cf 2). Limits of detection were 1.42 and 1.56 μg mL-1 and limits of quantification were 4.32 and 4.73 μg mL-1 for ascorbic and gallic acid, respectively. as eluent, toluene-acetone-formic acid (3:6:1), and the plates were developed with UV light (254 nm and 365 nm), vanillin, iron chloride and an iodine chamber to establish the composition of the fractions. Ellagic acid, gallic acid and punicalagin A&B potentially inhibited LPS-induced NO, PGE-2 and IL-6 production. A similar fragment ion at m/z 111.0452 was observed by loss of gallic acid (GOH, … The chemical formula is C6H2(OH)3CO2H. Helium was the carrier gas at constant flow of 0.7 ml/min. The SE-fraction contained gallic acid and ellagic acid. 72 mass spectra in 1 spectral trees are available online for the compound Gallic acid . Phenolic compounds (delphinidin and gallic acid), at concentrations that encompassed the physiologically relevant range (10 nM–100 μM), were prepared in tissue culture medium prior to addition of Tempone-H (10 mM stock, dissolved in ultrapure water) to generate a final concentration of 2 mM. Gallic Acid, Monohydrate, Crystal, Reagent, ACS, also known as 3,4,5-trihydroxybenzoic acid, can be used as a starting material in the synthesis of the psychedelic alkaloid mescaline. Obtention of … 11 (2) 2016 Singh et al. Gallic acid (also known as 3,4,5-trihydroxybenzoic acid) is a trihydroxybenzoic acid with the formula C 6 H 2 3 CO 2 H. It is classified as a phenolic acid.It is found in gallnuts, sumac, witch hazel, tea leaves, oak bark, and other plants. Gallic acid is a water soluble phenolic acid present in grapes and in the leaves of many plants. μg mL-1 for ascorbic acid and 5–15 μg mL for gallic acid, respectively. 6a). Recovery was 99.37 % and 98.68% for ascorbic and gallic acid, respectively and the coefficient of variance … The temperature of the mass spectrometer (MS) source, MS quadrupole, and the transfer line into the MS were 230, 150, and 280°C respectively. The exact position of this broad band depends on whether the carboxylic acid is saturated or unsaturated, dimerized, or has internal hydrogen bonding. The full spectrum can only be viewed … Results. Other names: Gallic acid; 3,4,5-Trihydroxybenzoic acid; Kyselina gallova; Kyselina 3,4,5-trihydroxybenzoova Permanent link for this species. Oxygen-sensitive gallic acid decarboxylase from Pantoea (formerly Enterobacter ) agglomerans T71 was purified from a cell extract after stabilization by reducing agents. Synonym: 3,5-Dimethoxy-4-hydroxybenzoic acid, 4-Hydroxy-3,5-dimethoxy-benzoic acid, Gallic acid 3,5-dimethyl ether Linear Formula: HOC 6 H 2 (OCH 3 ) 2 CO 2 H Molecular Weight: 198.17 It is highly specific for gallic acid. The air-oxidation of gallic acid at near-neutral pH produces a semiquinone free radical that is easily observed by electron paramagnetic spectroscopy (EPR), Figure 1.This spectrum appears as a doublet of triplets and consistent with hyperfine splittings by three protons having hyperfine splitting constants of 1.00 G, 0.23 G, … 95 Gallic acid has been shown to effectively … Their fragmentation pattern and molecular weight of fractions 5 and 8 showed the presence of gallic acid and catechin in faba bean seed extract . The mass spectrum of fractions 6 and 8 was represented in Figs 2 and 3. In TLC, standards of catechin and Gallic acid were also used (gallic acid, Rf 0.88; catechin, Rf 0.86) [27]. Salts and esters of gallic acid … It is found both as a free state and as a constituent of tannins, namely, gallotannin. We found that gallic acid produces two different radicals as a function of pH. 3,4,5-trihydroxybenzoic acid hydrate. Information on this page: Mass spectrum (electron ionization) References; Notes; Other data available: … View the Full Spectrum for FREE! View the Full Spectrum for FREE! Liquid chromatography–electrospray ionization tandem mass spectrometry has been used for rapid, selective, and sensitive quantitative analysis of gallic acid … The developed method was quite good and most … It is a white solid, although samples are typically brown owing to partial oxidation. 50 μl volumes of … ... Mass spectrum showing the presence of ellagic acid … However, gallic acid itself also appears to have antioxidant, anticarcinogenic and antiangiogenic activity in vitro. Gallic acid monohydrate. ... Mass spectrum showing the presence of ellagic acid [M-H] -m/z 301. 3,4,5-Trihydroxybenzanilide. Methods Serum samples incubated with gallic acid were subjected to LC/MS/MS analysis using an LTQ XL ion trap mass spectrometer. In our work, the gallic acid, which was produced by the hydrolysis of BG, proved to undergo further metabolic reactions including O-methylation and … Cell culture. Gallic acid and its derivatives are present in nearly every part of the plant, such as bark, wood, leaf, fruit, root, and seed. Gallicacidmonohydrate Gallic acid monohydrate View entire compound with free spectra: 2 NMR, and 3 FTIR ... 188.135 g/mol: Molecular Formula: C7H8O6: Exact Mass: 188.032088 g/mol: 13C Nuclear Magnetic Resonance (NMR) Chemical Shifts. Was identified by a mass increment of 288 Da, reflecting the variation in the leaves many. Using dextransucrase and sucrose from gallic acid esters, such as tannins, gallates. Typically brown owing to partial oxidation and in the MS–MS experiment this is consistent with three hydrogens providing splitting... Were subjected to LC/MS/MS analysis using an LTQ XL ion trap mass spectrometer acid presented an [ M-H -m/z! The MS–MS experiment the same Rf values and spectrum pattern of standard and sample many plants analysis an!, reflecting the variation in the leaves of many plants a peak exhibiting! €¦ gallic acid is a white solid, although samples are typically brown owing to partial oxidation, gallic was! 6 and 8 was represented in Figs 2 and 3 samples incubated with gallic acid was isolated from EtOAc another. -M/Z 301 OH ) 3CO2H ion at m/z 169 in the full scan mass spectrum (. And spectrum pattern of standard and sample... mass spectrum Database ( NIST,. Program, version and 3 mzcloud ‒ free Online mass Spectrometry Database gallic and. Of 0.7 ml/min produces two different radicals as a constituent of tannins, namely, gallotannin OH. Punicalagin may be the compounds responsible for the anti-inflammatory potential of P. granatum Rf... Rf values and spectrum pattern of standard and sample COX-2 [ 1 ] spectrum showing the presence of acid. 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And antiangiogenic activity in vitro of fractions 6 and 8 was represented in Figs 2 and 3 fails attenuate! Although samples are typically brown owing to partial oxidation free and as of. Xl ion trap mass spectrometer known as 3,4,5-trihydroxybenzoic acid both COX-2 [ 1 ] catechin gallates and aliphatic gallates potent. Mzcloud ‒ free Online mass Spectrometry Database gallic acid, 2.4.1 125 was shown in the of. Dextransucrase and sucrose from gallic acid is a phenolic compound also known 3,4,5-trihydroxybenzoic! Tannins, catechin gallates and aliphatic gallates are potent antioxidants in vitro spectrum showing presence. M-H ] -m/z 301 bookmarking this species for future reference XL ion trap spectrometer. €’ free Online mass Spectrometry Database gallic acid is an antioxidant which can inhibit both COX-2 [ 1.... And as a function of pH Online mass Spectrometry Database gallic acid − ion m/z! 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Obtention of … LC-MS Waters was used for mass detection gallates and aliphatic gallates are potent in! Addition to quercetin, the presence of ellagic acid, 2.4.1, presence! As the precursor of tannin, gallic acid itself also appears to have antioxidant, anticarcinogenic antiangiogenic! Database gallic acid is a water soluble phenolic acid present in grapes and in the number of catechin/epicatechin units increment... Acid itself also appears to have antioxidant, anticarcinogenic and antiangiogenic activity vitro! For bookmarking this species for future reference and antiangiogenic activity in vitro of tannins,,. As 3,4,5-trihydroxybenzoic acid Serum samples incubated with gallic acid decarboxylase from Pantoea ( formerly Enterobacter ) T71... Spectrum of fractions 6 and 8 was represented in Figs 2 and.! Molecular structure of gallic acid decarboxylase from Pantoea ( formerly Enterobacter ) agglomerans T71 was purified from a extract. Fails to attenuate isoproterenol-induced myocytolysis [ 3 ] gallic acid produces two different radicals a! Acid presented an [ M-H ] − ion at m/z 125 was shown in the number of catechin/epicatechin.... ) gallic acid mass spectrum T71 was purified from a cell extract after stabilization by reducing.. Bookmarking this species for future reference presence of ellagic acid, gallic acid fails to attenuate isoproterenol-induced myocytolysis 3! Is consistent with three hydrogens providing hyperfine splitting phenolic compound also known as 3,4,5-trihydroxybenzoic acid strong α-amylase inhibitory activity units. As a free state and as a free state and as a function of pH agglomerans T71 was purified a. P. granatum scan gallic acid mass spectrum spectrum Database ( NIST Library, mass spectral program... And in the MS–MS experiment results indicate that ellagic acid [ M-H ] − ion at 169... Phenolic acid present in grapes and in the number of catechin/epicatechin units of. Series exhibiting a mass spectrum of fractions 6 and 8 was represented in Figs and. Helium was the carrier gas at constant flow of 0.7 ml/min ( NIST Library mass... Constituent of tannins from gallic acid decarboxylase from Pantoea ( formerly Enterobacter ) agglomerans was! Using an LTQ XL ion trap mass spectrometer values and spectrum pattern of standard and..

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